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cd8 cd56 viable t cells (Miltenyi Biotec)

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Miltenyi Biotec cd8 cd56 viable t cells

Heterogenous alloresponse tested by in vitro mixed lymphocyte reaction (MLR)/flow cytometry assays in 63 responder/stimulator cell combinations that were characterized by a single HLA-C mismatch . The mismatched HLA-C allele of the stimulator cells and the %ΔCD137 + <t>CD8</t> + cells induced after restimulation at day 14 are indicated (see ). The cutoff of 2% ΔCD137 + CD8 + cells is indicated by the dashed line. Activation of CD8 + NK cells (ranging between 0.2 and 1.2% CD8 + <t>CD56</t> + CD137 + cells, results not shown) was not taken into consideration. Overall, 24 different shared HLA-A~B~DRB1~DQB1 haplotypes were tested (data not shown). Twelve MLRs were repeated but dotted as single mean %ΔCD137 + CD8 + values. Black dots represent MLRs with HLA-C MMs located in α1/α2 domains and HLA-DPB1 MMs. Blue triangles represent MLRs with HLA-C MMs located in α1/α2 domains and matched HLA-DPB1. Open circles represent MLRs with C*03:03/03:04 MMs and C*03:04/03:03 MMs, all HLA-DPB1 MMs. Open triangles represent MLRs with HLA-C MMs located outside α1/α2 domains (C*02:29/02:02, C*02:02/02:29) and matched HLA-DPB1.

Cd8 Cd56 Viable T Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 42 article reviews

cd8 cd56 viable t cells - by Bioz Stars, 2026-10

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1) Product Images from "Allorecognition of HLA-C Mismatches by CD8 + T Cells in Hematopoietic Stem Cell Transplantation Is a Complex Interplay between Mismatched Peptide-Binding Region Residues, HLA-C Expression, and HLA-DPB1 Disparities"

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2016.00584

Figure Legend Snippet: Heterogenous alloresponse tested by in vitro mixed lymphocyte reaction (MLR)/flow cytometry assays in 63 responder/stimulator cell combinations that were characterized by a single HLA-C mismatch . The mismatched HLA-C allele of the stimulator cells and the %ΔCD137 + CD8 + cells induced after restimulation at day 14 are indicated (see ). The cutoff of 2% ΔCD137 + CD8 + cells is indicated by the dashed line. Activation of CD8 + NK cells (ranging between 0.2 and 1.2% CD8 + CD56 + CD137 + cells, results not shown) was not taken into consideration. Overall, 24 different shared HLA-A~B~DRB1~DQB1 haplotypes were tested (data not shown). Twelve MLRs were repeated but dotted as single mean %ΔCD137 + CD8 + values. Black dots represent MLRs with HLA-C MMs located in α1/α2 domains and HLA-DPB1 MMs. Blue triangles represent MLRs with HLA-C MMs located in α1/α2 domains and matched HLA-DPB1. Open circles represent MLRs with C*03:03/03:04 MMs and C*03:04/03:03 MMs, all HLA-DPB1 MMs. Open triangles represent MLRs with HLA-C MMs located outside α1/α2 domains (C*02:29/02:02, C*02:02/02:29) and matched HLA-DPB1.

Techniques Used: In Vitro, Flow Cytometry, Activation Assay

Box and whisker plots of HLA-C mRNA expression quantified by RT-PCR in stimulator cells at day 0 inducing a negative versus a positive (cutoff = 2% ΔCD137 + CD8 + cells) alloresponse .

Figure Legend Snippet: Box and whisker plots of HLA-C mRNA expression quantified by RT-PCR in stimulator cells at day 0 inducing a negative versus a positive (cutoff = 2% ΔCD137 + CD8 + cells) alloresponse .

Techniques Used: Whisker Assay, Expressing, Reverse Transcription Polymerase Chain Reaction

Scatter plot of HLA-C expression [mean fluorescence intensity (MFI) values according to Ref. ( , )] and alloreactivity (%ΔCD137 + CD8 + cells) . A label is plotted for each stimulator allele at the top of the graph, indicating its corresponding MFI value. The linear regression through the data points is shown in red, and the confidence interval is in yellow (coefficient = 0.004, p = 0.637).

Figure Legend Snippet: Scatter plot of HLA-C expression [mean fluorescence intensity (MFI) values according to Ref. ( , )] and alloreactivity (%ΔCD137 + CD8 + cells) . A label is plotted for each stimulator allele at the top of the graph, indicating its corresponding MFI value. The linear regression through the data points is shown in red, and the confidence interval is in yellow (coefficient = 0.004, p = 0.637).

Techniques Used: Expressing, Fluorescence

Box and whisker plots of the number of mismatched residues at HLA-C between the stimulator and the responder alleles and alloreactivity defined as negative or positive (cutoff = 2% ΔCD137 + CD8 + cells) . The boxes correspond to the interquartile range, the median is the thick line inside the box, and whiskers extend up to observations that are outside the box for less than 1.5 times the interquartile range. No outliers to these limits were observed. The observations are also plotted individually with information on two predictor variables: matching at HLA-DPB1 is indicated by two different colors (blue for matched and red for mismatched HLA-DPB1), while variation at position 116 is indicated by the shape of the dots (a reversed triangle for matched and a circle for mismatched).

Figure Legend Snippet: Box and whisker plots of the number of mismatched residues at HLA-C between the stimulator and the responder alleles and alloreactivity defined as negative or positive (cutoff = 2% ΔCD137 + CD8 + cells) . The boxes correspond to the interquartile range, the median is the thick line inside the box, and whiskers extend up to observations that are outside the box for less than 1.5 times the interquartile range. No outliers to these limits were observed. The observations are also plotted individually with information on two predictor variables: matching at HLA-DPB1 is indicated by two different colors (blue for matched and red for mismatched HLA-DPB1), while variation at position 116 is indicated by the shape of the dots (a reversed triangle for matched and a circle for mismatched).

Techniques Used: Whisker Assay

Scatter plot of the number of mismatched residues at HLA-C between the stimulator and the responder allele and alloreactivity (%ΔCD137 + CD8 + cells) . Information on several predictor variables is plotted: matching at HLA-DPB1 is indicated by two different colors (blue for matched and orange for mismatched HLA-DPB1), mRNA expression of the stimulator HLA-C allele is indicated by the size of the dots, while variation at position 116 is indicated by the shape of the dots (a reversed triangle for matched and a circle for mismatched). The cutoff value considered for positive/negative alloreactivity is shown by the red dotted line.

Figure Legend Snippet: Scatter plot of the number of mismatched residues at HLA-C between the stimulator and the responder allele and alloreactivity (%ΔCD137 + CD8 + cells) . Information on several predictor variables is plotted: matching at HLA-DPB1 is indicated by two different colors (blue for matched and orange for mismatched HLA-DPB1), mRNA expression of the stimulator HLA-C allele is indicated by the size of the dots, while variation at position 116 is indicated by the shape of the dots (a reversed triangle for matched and a circle for mismatched). The cutoff value considered for positive/negative alloreactivity is shown by the red dotted line.

Techniques Used: Expressing

Mixed lymphocyte reactions (MLRs) between different responders and the same HLA-C MM stimulator . Each panel represents MLRs between cells of three (A) or two (B–D) responders and one stimulator. MLRs of each panel were done in parallel (i.e., at the same time) for each of the four experiments (A–D) and represent four different HLA-C MMs: the mismatched HLA-C alleles of the responder and the stimulator are indicated below each panel. Alloresponses are given as %ΔCD137 + CD8 + cells (2% cutoff indicated by the dashed line). All pairs tested in (A–C) were HLA-DPB1 mismatched. In experiment (D) , the two pairs (one positive, one negative) were HLA-DPB1 matched.

Figure Legend Snippet: Mixed lymphocyte reactions (MLRs) between different responders and the same HLA-C MM stimulator . Each panel represents MLRs between cells of three (A) or two (B–D) responders and one stimulator. MLRs of each panel were done in parallel (i.e., at the same time) for each of the four experiments (A–D) and represent four different HLA-C MMs: the mismatched HLA-C alleles of the responder and the stimulator are indicated below each panel. Alloresponses are given as %ΔCD137 + CD8 + cells (2% cutoff indicated by the dashed line). All pairs tested in (A–C) were HLA-DPB1 mismatched. In experiment (D) , the two pairs (one positive, one negative) were HLA-DPB1 matched.

Techniques Used:

Mixed lymphocyte reactions (MLRs) between different HLA-C MM stimulators and the same responder . Each panel represents MLRs between responder cells isolated from the same individual and HLA-C MM stimulator cells from two to three different individuals. MLRs of each panel were performed in parallel (i.e., at the same time) for each experiment and represent four different HLA-C MM: the mismatched HLA-C alleles of the responder and the stimulator are indicated below each panel. Alloresponses are given as %ΔCD137 + CD8 + cells (2% cutoff indicated by the dashed line). All pairs were DPB1 incompatible except two pairs [dashed bars in (A,C) ]. (E) shows the correlation between HLA-C mRNA expression of the stimulator cells and the induced alloresponse (%ΔCD137 + CD8 + cells): r = 0.42, p = 0.056. Triangles correspond to HLA-DPB1-matched pairs. All other MLRs were HLA-DPB1 mismatched. Colors in (E) correspond to those used in (A–D) .

Figure Legend Snippet: Mixed lymphocyte reactions (MLRs) between different HLA-C MM stimulators and the same responder . Each panel represents MLRs between responder cells isolated from the same individual and HLA-C MM stimulator cells from two to three different individuals. MLRs of each panel were performed in parallel (i.e., at the same time) for each experiment and represent four different HLA-C MM: the mismatched HLA-C alleles of the responder and the stimulator are indicated below each panel. Alloresponses are given as %ΔCD137 + CD8 + cells (2% cutoff indicated by the dashed line). All pairs were DPB1 incompatible except two pairs [dashed bars in (A,C) ]. (E) shows the correlation between HLA-C mRNA expression of the stimulator cells and the induced alloresponse (%ΔCD137 + CD8 + cells): r = 0.42, p = 0.056. Triangles correspond to HLA-DPB1-matched pairs. All other MLRs were HLA-DPB1 mismatched. Colors in (E) correspond to those used in (A–D) .

Techniques Used: Isolation, Expressing

Flow Cytometry:

Article Title: Allorecognition of HLA-C Mismatches by CD8 + T Cells in Hematopoietic Stem Cell Transplantation Is a Complex Interplay between Mismatched Peptide-Binding Region Residues, HLA-C Expression, and HLA-DPB1 Disparities
Article Snippet: After 13 days of culture, responding T cells were restimulated overnight with irradiated PKH-2 (Sigma-Aldrich, Buchs, Switzerland)-labeled PHA blasts obtained by activation of non-irradiated stimulatory PBMCs with 1 μg/ml PHA (Gibco). .. The percentage of CD137 + PKH-2 − CD8 + CD56 − viable T cells was quantified by flow cytometry using APC-labeled anti-human CD8a, PerCP/Cy5.5 anti-human CD56 (BioLegend, Fell, Germany), and FITC-labeled anti-human CD137 (Milteny Biotec, Bergisch-Gladbach, Germany) antibodies, as well as APC- and FITC-labeled murine IgG1 isotype controls (BD Bioscience, Allschwil, Switzerland). ..

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Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2016.00584

Figure Lengend Snippet: Heterogenous alloresponse tested by in vitro mixed lymphocyte reaction (MLR)/flow cytometry assays in 63 responder/stimulator cell combinations that were characterized by a single HLA-C mismatch . The mismatched HLA-C allele of the stimulator cells and the %ΔCD137 + CD8 + cells induced after restimulation at day 14 are indicated (see ). The cutoff of 2% ΔCD137 + CD8 + cells is indicated by the dashed line. Activation of CD8 + NK cells (ranging between 0.2 and 1.2% CD8 + CD56 + CD137 + cells, results not shown) was not taken into consideration. Overall, 24 different shared HLA-A~B~DRB1~DQB1 haplotypes were tested (data not shown). Twelve MLRs were repeated but dotted as single mean %ΔCD137 + CD8 + values. Black dots represent MLRs with HLA-C MMs located in α1/α2 domains and HLA-DPB1 MMs. Blue triangles represent MLRs with HLA-C MMs located in α1/α2 domains and matched HLA-DPB1. Open circles represent MLRs with C*03:03/03:04 MMs and C*03:04/03:03 MMs, all HLA-DPB1 MMs. Open triangles represent MLRs with HLA-C MMs located outside α1/α2 domains (C*02:29/02:02, C*02:02/02:29) and matched HLA-DPB1.

Article Snippet: The percentage of CD137 + PKH-2 − CD8 + CD56 − viable T cells was quantified by flow cytometry using APC-labeled anti-human CD8a, PerCP/Cy5.5 anti-human CD56 (BioLegend, Fell, Germany), and FITC-labeled anti-human CD137 (Milteny Biotec, Bergisch-Gladbach, Germany) antibodies, as well as APC- and FITC-labeled murine IgG1 isotype controls (BD Bioscience, Allschwil, Switzerland).

Techniques: In Vitro, Flow Cytometry, Activation Assay

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2016.00584

Figure Lengend Snippet: Box and whisker plots of HLA-C mRNA expression quantified by RT-PCR in stimulator cells at day 0 inducing a negative versus a positive (cutoff = 2% ΔCD137 + CD8 + cells) alloresponse .

Techniques: Whisker Assay, Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2016.00584

Figure Lengend Snippet: Scatter plot of HLA-C expression [mean fluorescence intensity (MFI) values according to Ref. ( , )] and alloreactivity (%ΔCD137 + CD8 + cells) . A label is plotted for each stimulator allele at the top of the graph, indicating its corresponding MFI value. The linear regression through the data points is shown in red, and the confidence interval is in yellow (coefficient = 0.004, p = 0.637).

Techniques: Expressing, Fluorescence

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2016.00584

Figure Lengend Snippet: Box and whisker plots of the number of mismatched residues at HLA-C between the stimulator and the responder alleles and alloreactivity defined as negative or positive (cutoff = 2% ΔCD137 + CD8 + cells) . The boxes correspond to the interquartile range, the median is the thick line inside the box, and whiskers extend up to observations that are outside the box for less than 1.5 times the interquartile range. No outliers to these limits were observed. The observations are also plotted individually with information on two predictor variables: matching at HLA-DPB1 is indicated by two different colors (blue for matched and red for mismatched HLA-DPB1), while variation at position 116 is indicated by the shape of the dots (a reversed triangle for matched and a circle for mismatched).

Techniques: Whisker Assay

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2016.00584

Figure Lengend Snippet: Scatter plot of the number of mismatched residues at HLA-C between the stimulator and the responder allele and alloreactivity (%ΔCD137 + CD8 + cells) . Information on several predictor variables is plotted: matching at HLA-DPB1 is indicated by two different colors (blue for matched and orange for mismatched HLA-DPB1), mRNA expression of the stimulator HLA-C allele is indicated by the size of the dots, while variation at position 116 is indicated by the shape of the dots (a reversed triangle for matched and a circle for mismatched). The cutoff value considered for positive/negative alloreactivity is shown by the red dotted line.

Techniques: Expressing

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2016.00584

Figure Lengend Snippet: Mixed lymphocyte reactions (MLRs) between different responders and the same HLA-C MM stimulator . Each panel represents MLRs between cells of three (A) or two (B–D) responders and one stimulator. MLRs of each panel were done in parallel (i.e., at the same time) for each of the four experiments (A–D) and represent four different HLA-C MMs: the mismatched HLA-C alleles of the responder and the stimulator are indicated below each panel. Alloresponses are given as %ΔCD137 + CD8 + cells (2% cutoff indicated by the dashed line). All pairs tested in (A–C) were HLA-DPB1 mismatched. In experiment (D) , the two pairs (one positive, one negative) were HLA-DPB1 matched.

Techniques:

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2016.00584

Figure Lengend Snippet: Mixed lymphocyte reactions (MLRs) between different HLA-C MM stimulators and the same responder . Each panel represents MLRs between responder cells isolated from the same individual and HLA-C MM stimulator cells from two to three different individuals. MLRs of each panel were performed in parallel (i.e., at the same time) for each experiment and represent four different HLA-C MM: the mismatched HLA-C alleles of the responder and the stimulator are indicated below each panel. Alloresponses are given as %ΔCD137 + CD8 + cells (2% cutoff indicated by the dashed line). All pairs were DPB1 incompatible except two pairs [dashed bars in (A,C) ]. (E) shows the correlation between HLA-C mRNA expression of the stimulator cells and the induced alloresponse (%ΔCD137 + CD8 + cells): r = 0.42, p = 0.056. Triangles correspond to HLA-DPB1-matched pairs. All other MLRs were HLA-DPB1 mismatched. Colors in (E) correspond to those used in (A–D) .

Techniques: Isolation, Expressing

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cd8 cd56 viable t cells (Miltenyi Biotec)

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Images

1) Product Images from "Allorecognition of HLA-C Mismatches by CD8 + T Cells in Hematopoietic Stem Cell Transplantation Is a Complex Interplay between Mismatched Peptide-Binding Region Residues, HLA-C Expression, and HLA-DPB1 Disparities"

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